40 nm Gold Nanospheres for Conjugation
Customers outside of the US & Canada: please visit your local distributor’s website to purchase this item using its SKU, or contact us here. Thank you!
40 nm spherical gold nanoparticles for conjugation are useful in applications such as immunodiagnostics, lateral flow assays, nanomedicine, drug delivery, and biosensors.
BioReady™ 40 nm Gold Nanospheres are available with the following surfaces:
- Citrate - for passive adsorption
- Carboxyl - for covalent conjugation
- Streptavidin - for high-affinity binding to biotin-labeled molecules
- Protein G - for oriented, Fc-specific affinity binding of IgG antibodies
Review our conjugation protocols below or contact our experts for support.
nanoComposix provides high quality, monodisperse, and aggregation-free nanomaterials. All our products are supplied with a batch-specific Certificate of Analysis including characterization data such as TEM, DLS, Zeta, and UV-Vis.
Intended for Research Use Only. Not for use in diagnostic procedures. Contact us for custom formulations, ISO 13485/cGMP compliant materials, or for bulk supply requirements.
| Overview | ||||
|---|---|---|---|---|
| Surface | Citrate | Carboxyl | Streptavidin | Protein G |
| Description | For passive adsorption | For covalent conjugation | For streptavidin-biotin binding | For oriented IgG binding |
| Solvent | 0.02 mM sodium citrate | USP purified water | 1X PBS, 0.5% BSA, and 0.05% azide | 1X PBS, 0.5% BSA, and 0.05% azide |
| Surface chemistry | Citrate | PEG12-carboxylic acid | Streptavidin | Protein G |
| Specifications | ||||
| TEM Diameter | 40 ± 4 nm | 40 ± 4 nm | 40 ± 4 nm | 40 ± 4 nm |
| CV | ≤ 15% | ≤ 15% | ≤ 15% | ≤ 15% |
| Documents | ||||
| Example Certificate of Analysis (CoA) |
20 OD (BioReady) 1 OD (BioReady) |
Download | Download | Download |
| Safety Data Sheet (SDS) |
20 OD (BioReady) 1 OD (BioReady) |
20 OD (BioReady) | 10 OD (BioReady) | 10 OD (BioReady) |
| Storage & Handling | Download | Download | Download | Download |
| Protocols |
Antibody Purification Passive Adsorption Protocol Passive Adsorption Tutorial Video↗ |
Antibody Purification Covalent Conjugation Protocol Covalent Conjugation Tutorial Video ↗ |
Antibody Purification Streptavidin Gold Conjugation Protocol |
Antibody Purification |
| Expected Ranges | ||||
| Hydrodynamic diameter (DLS) | 40-50 nm | 40-70 nm | 50-75 nm | 50-75 nm |
| Zeta potential | ≤ –25 mV | ≤ –25 mV | ≤ –10 mV | ≤ –10 mV |
| pH of solution | 5.5–8.0 | 5.5–7.0 | 6.8–8.2 | 6.8–8.2 |
| Peak wavelength (λmax) | 520–550 nm | 520–550 nm | 520–530 nm | 520–530 nm |