7 Products
Case Studies
Nutraceutical Particle Characterization
nanoComposix has over five years of experience characterizing nutraceutical compounds by Dynamic Light Scattering (DLS). DLS, which measures the Brownian motion particles in solution, was utilized to monitor the size of particle formulations in the nanometer to micrometer size range. DLS provided useful information to help researchers and manufacturers assess formulation stability, bioavailability, and dispersion of the oil phases and was used as a metric for quality control efforts.
Nanomedicine Pre-GMP Method Verification and DLS Characterization
nanoComposix used DLS as a pre-GMP method verification characterization step to verify the size of iron oxide nanobeads and to test measurement precision between different instruments and sample lots. Iron oxide nanobeads utilize magnetic properties to enhance blood diffusion when diagnosing and treating complex vascular blockages. The DLS verification was important because the microbeads presented a challenge to measure accurately using DLS due to magnetic properties that encourage agglomeration by design. Method verification was important to the client to improve the characterization of the product and for the future development of an analytical technique to repeatedly and accurately measure the microbeads.
Isoelectric Point Determination with Zeta Potential Analysis
nanoComposix has tested the stability of novel nanoparticles engineered by academic and industrial manufacturers by measuring the zeta potential of colloidal nanoparticles tuned to a range of specific pH measurements. This is used to determine the isoelectric point of the material, and thus pH range in which the nanoparticles will be unstable. The isoelectric point can also be used to determine the extent of surface functionalization, as it will shift based on the type and magnitude of ligand functionalization.
Pharmacokinetics and Biodistribution Studies using ICP-MS
ICP-MS is a valuable tool for analyzing the circulation kinetics of metallic colloidal nanoparticles in animal blood for pharmacokinetic studies. It has also been used in biodistribution studies for in vivo dosing in animal models, therapeutic research, and oncology investigations. The nanoComposix Characterization Services team has conducted numerous pharmacokinetics and biodistribution studies for various clients, testing thousands of samples through ICP-MS characterization.
Endotoxin Analysis of Nanoparticles and Biological Samples
The endotoxin concentration in nanoparticles and other biological samples such as antibodies, DNA, proteins, or peptides, can be precisely measured using the kinetic turbidimetric assay, which measures the reaction between endotoxin and a lysate. At nanoComposix, the Kinetic Turbidity LAL Assay is used and the NCL Method STE-1.2 is followed. Endotoxin analysis characterization offered by nanoComposix can be utilized for R&D or pre-clinical testing of samples from in-vivo animal studies.