BioReady nanoparticles are optimized for conjugation and lateral flow applications. If you need maximum sensitivity and conjugate stability, use gold nanoshells. For increased conjugate stability and reduced antibody costs compared to passive conjugation, use carboxyl gold nanospheres for covalent conjugation. For the lowest cost and highest performance when performing passive conjugation, use our carbonate or citrate gold nanospheres.
Ultra-bright gold nanoshells provide up to a 20× increase in lateral flow diagnostic sensitivity over 40 nm gold nanoparticles. Combining the benefits of gold nanoshell brightness and covalent conjugate stability, gold nanoshells are recommended for achieving maximum assay sensitivity. The Covalent Conjugate Optimization Kit includes everything needed to rapidly optimize your assay with carboxyl gold nanoshells. For rapid screening of antibody pairs, the NHS functionalized nanoshells are the fastest and simplest way to fabricate conjugates. Streptavidin nanoshells can be used to bind to biotin-containing molecules.
Gold nanoshells consist of a 120 nm silica core coated with a 15 nm thick gold shell. Due to the plasmon resonance of the gold nanoshells, an intense blue line will be visible on white lateral flow test strips.
Everything you need to get started conjugating to carboxyl gold nanoshells! Includes validated reagents and detailed protocols to jumpstart your optimization. Great for both beginners and experts at covalent EDC/NHS conjugation.
Antibodies and other amine-containing biomolecules efficiently bind to carboxyl gold nanoshells using EDC-NHS chemistry to make highly stable conjugates. Carboxyl gold nanoshells are provided as a 20 OD solution.
NHS-activated dried gold nanoshells are provided in a kit for rapid conjugations and antibody sweeps. No EDC/NHS activation required – just add purified antibody and follow our simple protocol.
Streptavidin is covalently bound to the gold nanoshell surface. Streptavidin gold conjugates bind to biotinylated antibodies, proteins, or oligonucleotides.
Extensively characterized and highly reproducible BioReady 40 and 80 nm gold nanoparticles are great for reliable covalent and passive conjugates. Covalent conjugates have increased stability in difficult sample matrices and can reduce overall assay costs by minimizing the amount of antibody needed per strip. A novel “bare” carbonate surface increases the performance of passive conjugation.
Covalent binding chemistry increases conjugate stability in a variety of matrices and allows greater control over per-particle antibody loading compared to passive conjugation. Covalent conjugation reduces antibody cost per strip and decreases development time. Carboxyl gold nanospheres are the most economical and highest performance covalent option for 40 and 80 nm gold. NHS gold nanospheres are useful for rapidly screening antibodies. For use with biotinylated reagents, use streptavidin nanospheres.
Antibodies and other amine-containing biomolecules efficiently bind to carboxyl gold nanoparticles using EDC-NHS chemistry. Carboxyl gold nanoparticles are provided as a 20 OD solution.
NHS-activated dried gold nanoparticles are provided in a kit for rapid conjugations and antibody sweep. No EDC/NHS activation required – just add purified antibody and follow our simple protocol.
Streptavidin is covalently bound to the gold nanoparticle surface. Streptavidin gold conjugates bind to biotinylated antibodies, proteins or oligonucleotides.
Gold nanoparticles with a “bare” surface are necessary for efficient passive conjugation. BioReady carbonate gold nanospheres have a highly displaceable surface that can improve the performance of passive conjugates. BioReady citrate gold nanospheres are a high quality, reproducible, and low-cost option for anyone seeking an alternative to gold nanoparticles they currently purchase or make in-house.
Carbonate is a smaller, less complex molecule with a lower affinity to the gold nanoparticle surface than typical stabilizing molecules (e.g., citrate). Many of our customers have doubled their sensitivity using carbonate gold nanoparticles.
Citrate is the most common stabilizer used for gold nanoparticles. BioReady citrate gold nanoparticles are highly characterized, have low lot-to-lot variability, and are very competitively priced.