20 nm Gold Nanospheres for Conjugation
Customers outside of the US & Canada: please visit your local distributor’s website to purchase this item using its SKU, or contact us here. Thank you!
20 nm spherical gold nanoparticles for conjugation are useful in applications such as biosensors, immunodiagnostics, and lateral flow assays.
BioReady™ 20 nm Gold Nanospheres are available with the following surfaces:
- Carboxyl - for covalent conjugation
- Citrate - for passive adsorption
- Streptavidin - for high-affinity binding to biotin-labeled molecules
Review our conjugation protocols below or contact our experts for support.
nanoComposix provides high quality, monodisperse, and aggregation-free nanomaterials. All our products are supplied with a batch-specific Certificate of Analysis including characterization data such as TEM, DLS, Zeta, and UV-Vis.
Intended For Research Use Only. Not for use in diagnostic procedures. Contact us for custom formulations, ISO 13485/cGMP compliant materials, or bulk supply requirements.
| Overview | |||
|---|---|---|---|
| Surface | Carboxyl | Citrate | Streptavidin |
| Description | For covalent conjugation | For passive adsorption | For streptavidin-biotin binding |
| Solvent | USP purified water | 0.02 mM sodium citrate | 1X PBS, 0.5% BSA, and 0.05% azide |
| Surface chemistry | PEG12-carboxylic acid | Citrate | Streptavidin |
| Specifications | |||
| TEM Diameter | 20 ± 3 nm | 20 ± 3 nm | 20 ± 3 nm |
| CV | ≤ 15% | ≤ 15% | ≤ 15% |
| Documents | |||
| Example Certificate of Analysis (CoA) | Download |
1 OD 20 OD |
10 OD |
| Safety Data Sheet (SDS) | 20 OD |
1 OD 20 OD |
10 OD |
| Storage & Handling | Download | Download | Download |
| Protocols |
Antibody Purification Covalent Conjugation Protocol Covalent Conjugation Tutorial Video ↗ |
Antibody Purification Passive Adsorption Protocol Passive Adsorption Tutorial Video↗ |
Antibody Purification Streptavidin Conjugation Protocol |
| Expected Ranges | |||
| Hydrodynamic diameter (DLS) | 20-50 nm | 20-30 nm | 20-50 nm |
| Zeta potential | ≤ –25 mV | ≤ –25 mV | ≤ –10 mV |
| pH of solution | 6.5–7.0 | 5.5–8.5 | 6.8–8.2 |
| Peak wavelength (λmax) | 515–560 nm | 515–560 nm | 515–560 nm |