Covalent Conjugation Kit – BioReady Gold Nanoshells
Customers outside of the US & Canada: please visit your local distributor’s website to purchase this item using its SKU, or contact us here. Thank you!
Imagine an optimized system with time-after-time consistency that provides increased sensitivity and saves you time and money!
The High Sensitivity Conjugation Kit is a complete “classroom-in-a-box” with detailed protocols to help you produce robust covalently-bound conjugates. This kit is available with our ultra-bright 150 nm gold nanoshells as the reporter particle and includes everything you need to optimize your conjugations.
Benefits of the kit include:
- Pre-validated consumables eliminate the risk of purchasing non-compatible reagents, saving weeks to months of unnecessary troubleshooting
- Increase your assay sensitivity up to 20-fold with 150 nm gold nanoshells compared to 40 nm gold* nanoparticles
- Reduces antibody costs up to 65% compared to physisorption
- Saves time by reducing the number of conjugations necessary to find optimal antibody pairs
- Increases conjugate stability in a wide range of sample matrices
- Eliminates the need for repeated pH and salt sweeps for each new lot of gold nanoparticles
- Precisely controls the number of antibodies on the particle surface and extends the dynamic range in competitive assay formats
- Includes answers to frequently asked questions and troubleshooting tips to help you rapidly succeed in your diagnostic development projects
Already a conjugation expert? This kit eliminates the need to pre-screen and validate critical reagents and supplies to ensure your best chance of success when starting out with covalent conjugation to gold nanoshells*.
*Interested in a 40 nm or 80 nm gold kit? Get all the benefits of robust covalent conjugates and a kit with pre-validated reagents and protocols here.
Kit includes 10 mL of BioReady 150 nm Gold Nanoshells with a carboxyl surface, provided at 20 OD. See protocol for list of other buffers and reagents provided.
Overview | |
---|---|
Surface | Carboxyl |
Description | Covalently bound. Conjugatable surface. |
Solvent | 0.02 mM potassium carbonate |
Surface chemistry | Lipoic acid |
Specifications | |
TEM Diameter | 155 ± 15 nm |
CV | ≤ 15% |
Documents | |
Example Certificate of Analysis (CoA) | Download |
Safety Data Sheet (SDS) | 20 OD (BioReady) |
Storage & Handling | Download |
Protocols | Antibody Purification Kit Components High Sensitivity Conjugation Kit Protocol Covalent Conjugation Tutorial Video ↗ |
Expected Ranges | |
Hydrodynamic diameter (DLS) | 145–195 nm |
Zeta potential | ≤ –40 mV |
pH of solution | 6.0–9.5 |
Peak wavelength (λmax) | 770–840 nm |